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IGCSE · Cambridge (CIE) · 0973

Distinguish turgid flaccid and plasmolysed plant cells

Explain changes in water potential, turgor pressure and membrane position.

Section B3.2 · Osmosis

Osmosis · Cambridge IGCSE 0973 · B3.2
Exam scope and assessment notes

Mapped to the named 0973 section using teaching from the corresponding single-science course. A 0973-specific review of every Core and Supplement objective is still in progress. Written practice is self-checked; it does not demonstrate supervised laboratory competence or award an official Cambridge grade. This qualification reports two grades on the 9–1 scale; confirm the entry route for your zone and exam series.

Before you begin

  • Recall water potential and supporting walls.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

What directly indicates plasmolysis in the model?

What you will learn

  • Explain changes in water potential, turgor pressure and membrane position.
  • Apply the idea to a supplied biological example or investigation.
Schematic turgid, flaccid and plasmolysed plant cells retain the same wall outline. Turgor is high in the turgid state, lower in the flaccid state; visible membrane separation from the wall identifies plasmolysis.Keep the wall, change thecontentsTurgidHigh turgorFlaccidLow turgorPlasmolysedSeparation
Schematic turgid, flaccid and plasmolysed plant cells retain the same wall outline. Turgor is high in the turgid state, lower in the flaccid state; visible membrane separation from the wall identifies plasmolysis.

Describe the cell states

A turgid plant cell contains enough water for its contents to exert substantial outward turgor pressure against the wall. Water entering from higher external water potential increases this pressure; the wall resists expansion. The pressure increase can reduce the overall water-potential difference and limit further net entry.

A flaccid cell has reduced turgor pressure and is less firm. Losing some water does not automatically mean its membrane has visibly pulled away from the wall. Turgid and flaccid describe pressure/support states, not whether the wall exists.

Recognise plasmolysis

In sufficiently low external water potential, water leaves by osmosis, the vacuole and cytoplasm shrink, and the cell membrane can pull away from the cell wall. This separation is plasmolysis. The wall remains outside; it does not shrink inward with the membrane in this model.

Not every wilted tissue is visibly plasmolysed. A supplied microscope observation of membrane separation supports that label more directly than mass loss alone. Replacing a concentrated surrounding solution with a more suitable dilute solution may reverse separation in living, undamaged cells, but recovery is not guaranteed for damaged tissue.

Sources for this lesson

Pause and explain

What is turgor pressure?

Put the idea to work

Worked example

A plant micrograph shows the membrane and contents pulled inward from an unchanged cell wall after immersion. Identify the state.

Show the worked solution
  1. The wall remains outside.
  2. The membrane has separated from it.
  3. This is stronger evidence than tissue mass loss alone.

Answer Plasmolysis.

Try a topic test

Choose Easy, Normal or Difficult. Save your answers and open worked review after finishing.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Explain why a turgid plant cell is firm.

Give me a hint

Connect water, pressure and wall.

Compare my reasoning
  1. Water-filled contents press outward.
  2. The wall resists expansion.

Turgor pressure against the resisting wall gives firmness.

Look for these in your work

  • Use turgor pressure correctly.
2 · Independent

A cell loses some water and has low turgor, but no visible membrane separation. Which term fits best?

Give me a hint

Do not overclaim plasmolysis.

Compare my reasoning
  1. Pressure has fallen.
  2. Separation is not observed.

Flaccid.

Look for these in your work

  • Distinguish the two water-loss states.
3 · Transfer

Explain a membrane pulling away from the wall in a concentrated external solution.

Give me a hint

Use water potential and osmosis.

Compare my reasoning
  1. Outside water potential is lower in the stated model.
  2. Water leaves; vacuole and cytoplasm shrink.
  3. The membrane separates from the unchanged wall.

Water loss by osmosis causes plasmolysis.

Look for these in your work

  • Link gradient, water loss and membrane position.

Common mistakes

  • Flaccid does not automatically mean visibly plasmolysed.
  • The wall does not pull inward with the membrane.
Recall without your notes

What can you explain now?

What is turgor pressure?

Compare with the explanation

Outward pressure of cell contents against the wall.

It contributes to plant firmness.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Sketch the same plant cell in three states and keep the wall fixed.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Sciences — Co-ordinated (9-1)

Course scope and assessment

This 9–1 pathway maps all 152 detailed subject-content sections in the published 0973 syllabus for exams in 2028 and 2029. The course map follows the same 2028–2029 section codes as Cambridge's A*–G 0654 syllabus, while keeping 0973's grading and entry rules separate. It adapts lessons from the related single-science courses and excludes clear single-science-only topics. A 0973-specific objective-by-objective review, Core/Supplement audit, human subject-teacher review, supervised laboratory experience and cumulative assessment remain in progress. Written practice is self-checked and does not predict an official grade. The separate 2025–2027 syllabus has not been reconciled here. Cambridge lists 0973 only in a limited number of administrative zones: June in zone 3 and centres in Oman and the UAE; November in zone 3 only. Confirm entry with your centre.

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