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A Level · Cambridge (CIE) · 9700

Distinguish cyclic and non-cyclic photophosphorylation

State that both cyclic and non-cyclic photophosphorylation occur in the light-dependent stage and compare their outcomes.

Section 13.1 · Photosynthesis as an energy transfer process

Photosynthesis as energy transfer pathway · 13.1

A Level; Cambridge International Biology 9700, 2028–2030. Written practice is self-checked; practical investigations need supervised work and human subject-teacher review remains pending.

Before you begin

  • Chloroplasts contain photosynthetic pigments, and enzymes catalyse metabolic reactions.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

Which route uses both PSI and PSII?

What you will learn

  • Name cyclic and non-cyclic photophosphorylation
  • Compare photosystems and products at syllabus level

Build the explanation

Photophosphorylation is ATP synthesis during the light-dependent stage. It occurs in two forms: cyclic and non-cyclic. Cyclic photophosphorylation involves photosystem I (PSI) and makes ATP. Non-cyclic photophosphorylation involves both PSI and photosystem II (PSII), photolysis of water by the oxygen-evolving complex, and production of ATP and reduced NADP; oxygen is released from water. The routes therefore differ in photosystems and products.

Apply it carefully

Do not infer that cyclic photophosphorylation releases oxygen or forms reduced NADP in the syllabus model. Both routes can synthesize ATP; only the non-cyclic route described here includes PSII and water photolysis, producing reduced NADP and oxygen.

Pause and explain

Which photophosphorylation route releases oxygen from water?

Put the idea to work

Worked example

Which route uses PSI only and synthesizes ATP?

Show the worked solution
  1. Compare the photosystems involved.
  2. Cyclic photophosphorylation uses PSI only.

Answer Cyclic photophosphorylation.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Compare cyclic and non-cyclic photophosphorylation by photosystems and products.

Give me a hint

Identify which one includes PSII and water photolysis.

Compare my reasoning
  1. Cyclic: PSI only; ATP is synthesised.
  2. Non-cyclic: PSI and PSII; water is split and ATP and reduced NADP are synthesised, with oxygen released.

Cyclic photophosphorylation involves PSI only and synthesises ATP. Non-cyclic photophosphorylation involves PSI and PSII; water photolysis supplies electrons and produces oxygen, and ATP and reduced NADP are synthesised.

Look for these in your work

  • Photosystems compared
  • ATP in both stated
  • Non-cyclic products stated
2 · Independent

A sample produces ATP but no detectable oxygen or reduced NADP during the tested light response. Which route is more consistent with the syllabus model, and what cannot be concluded from this evidence alone?

Give me a hint

Use the product pattern but consider detection limits and other conditions.

Compare my reasoning
  1. ATP without the two non-cyclic products is consistent with cyclic photophosphorylation.
  2. The evidence does not prove that no non-cyclic activity occurred if product detection or conditions were limited.

The pattern is consistent with cyclic photophosphorylation, which uses PSI and synthesises ATP. It does not prove that non-cyclic activity is absent unless the detection method and conditions are known to detect its products reliably.

Look for these in your work

  • Likely route identified
  • Evidence limit considered
  • No absolute claim
3 · Transfer

A learner says cyclic photophosphorylation must replace non-cyclic photophosphorylation whenever light intensity falls. Correct this inference.

Give me a hint

The route definitions do not specify a universal switching rule.

Compare my reasoning
  1. Cyclic and non-cyclic routes differ in photosystems and products.
  2. A change in light intensity alone does not establish which route replaces the other without more conditions or evidence.

Cyclic and non-cyclic photophosphorylation are distinct light-dependent routes with different photosystem involvement and products. The stated syllabus outcomes do not imply that lower light automatically switches one route for the other; additional conditions and evidence are needed.

Look for these in your work

  • Route difference retained
  • Unsupported automatic switch rejected
  • Evidence need stated

Common mistakes

  • Saying cyclic photophosphorylation uses PSII
  • Assigning oxygen release to the cyclic route
  • Treating ATP as unique to non-cyclic photophosphorylation
Recall without your notes

What can you explain now?

Which route uses both PSI and PSII?

Compare with the explanation

Non-cyclic photophosphorylation

The non-cyclic route also involves water photolysis and reduced NADP production.

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Make it stick

Which photosystems and products distinguish cyclic from non-cyclic photophosphorylation?

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Keep learning Biology

Course scope and assessment

Two hundred and fifty-nine new lessons map every published learning outcome in Cambridge International Biology 9700 Topics 1–19 for exams 2028–2030. Each topic lesson includes guided, independent and transfer practice. Fifteen additional practical-assessment lessons provide online preparation for Paper 3 and Paper 5 practical assessment skills. Online Paper 3 lessons cannot provide supervised laboratory competence. Full timed cumulative exam practice and human subject-teacher review remain outstanding, so this is not exam-readiness evidence.

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