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IGCSE · Cambridge (CIE) · 0610

Investigate temperature and photosynthetic gas release

Describe a tested temperature response with matched light and carbon dioxide.

Section 6.1 · Photosynthesis

Photosynthesis pathway · 6.1

Core and Extended; Biology 0610, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked

Before you begin

  • Recall chloroplasts, enzymes and the difference between photosynthesis and respiration.
  • Use the stated observations, quantities and controls; diagrams are schematic.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

Which measured temperature has the greatest supplied rate?

What you will learn

  • Describe a tested temperature response with matched light and carbon dioxide.
  • Use the stated evidence to explain the method and its limits.
Supplied gas-release rates at 10, 20, 30 and 40 °C are 0.2, 0.4, 0.6 and 0.1 cm³/min. The greatest measured rate is at 30 °C; additional nearby temperatures are needed to estimate an optimum. Joined points guide the eye and do not describe every plant.Fastest tested is not auniversal optimumGas-release rate (cm³/min)00.20.40.610203040Temperature (°C)30 °C: fastest testedExact optimum needs moresamples
Supplied gas-release rates at 10, 20, 30 and 40 °C are 0.2, 0.4, 0.6 and 0.1 cm³/min. The greatest measured rate is at 30 °C; additional nearby temperatures are needed to estimate an optimum. Joined points guide the eye and do not describe every plant.

Keep collection conditions controlled

Compare an aquatic plant at measured water temperatures using teacher-approved controlled baths. Match light, carbon dioxide treatment, plant quantity, adjustment time and gas collection duration. Check the actual water temperature rather than assuming a bath label is exact.

Use repeats and comparable fresh material if earlier heat exposure could cause lasting damage. Changes in water temperature can also affect gas solubility and collection, so gas-release measurements need careful interpretation.

Read the tested response

In the supplied example, gas-release rates at 10, 20, 30 and 40 °C are 0.2, 0.4, 0.6 and 0.1 cm³/min. The fastest tested setting is 30 °C, followed by a decline. Temperatures between those points have not been measured.

Photosynthesis involves enzyme-controlled reactions. A moderate rise can support faster reaction rates; excessive heat can disrupt enzyme function and damage tissues. These links help interpretation, but a decline in collection alone does not demonstrate that every enzyme has been denatured or establish a universal optimum for all plants.

Pause and explain

Why could reusing a previously overheated plant weaken a later low-temperature comparison?

Put the idea to work

Worked example

Use the supplied 10–40 °C rates to state the response and fastest tested setting.

Show the worked solution
  1. Rates rise from 0.2 to 0.6 cm³/min between 10 and 30 °C.
  2. They fall to 0.1 cm³/min at 40 °C.
  3. 30 °C is fastest tested, not a precisely established universal optimum.

Answer The tested response rises to 30 °C then falls; 30 °C has the highest measured rate.

Try a topic test

Choose Easy, Normal or Difficult. Save your answers and open worked review after finishing.

Test this topic →

From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Calculate the average rate when 2.4 cm³ gas is collected in 4 min at a tested temperature.

Give me a hint

Use gas volume divided by time.

Compare my reasoning
  1. 2.4 ÷ 4 = 0.6.
  2. Use cm³/min.

0.6 cm³/min.

Look for these in your work

  • Correct arithmetic.
  • Correct units.
2 · Independent

Compare supplied rates of 0.6 cm³/min at 30 °C and 0.1 cm³/min at 40 °C. State the ratio and absolute fall.

Give me a hint

Use both a ratio and a difference.

Compare my reasoning
  1. 0.6 ÷ 0.1 = 6.
  2. 0.6 − 0.1 = 0.5 cm³/min.

The 30 °C rate is six times the 40 °C rate; the fall is 0.5 cm³/min.

Look for these in your work

  • Distinguish ratio from rate difference.
  • Include units on the difference.
3 · Transfer

A single 30 °C result is high. Explain why it is not enough to establish the exact optimum temperature.

Give me a hint

Consider repeats and untested temperatures.

Compare my reasoning
  1. One result may vary.
  2. Nearby temperatures have not been tested closely.
  3. Repeat and add suitable nearby settings under control.

Repeat measurements and closer temperature sampling are needed; the supplied result identifies only a promising tested setting.

Look for these in your work

  • Name both reliability and sampling.
  • Avoid a universal optimum.

Common mistakes

  • Do not convert fastest tested into an exact optimum.
  • Gas collection and irreversible heat damage need separate checks.
Recall without your notes

What can you explain now?

Why could reusing a previously overheated plant weaken a later low-temperature comparison?

Compare with the explanation

Earlier heat may have lasting effects.

The new treatment could include a history effect.

After trying it yourself, choose your next review. This is your self-assessment.

Your review choice appears on Today. Sign in to sync it across devices.

Make it stick

Explain the tested temperature pattern and two ways to strengthen the comparison.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked.

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