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A Level · Cambridge (CIE) · 9700

Explain ABA-mediated stomatal closure

Trace how abscisic acid and calcium ions contribute to guard-cell responses during water stress.

Homeostasis in plants pathway · 14.2

A Level; Cambridge International Biology 9700, 2028–2030. Written answers are self-checked; this is not a practical skills certification.

Before you begin

  • A control system detects a change, communicates information and changes an effector response.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

Which signal increases during water stress to promote stomatal closure?

What you will learn

  • Describe the role of abscisic acid during water stress
  • Explain how calcium ions act as a second messenger in guard cells

Guard cells around a stomatal pore

A stoma is a pore between two guard cells. Changes in turgor alter the aperture; opening supports carbon-dioxide uptake but also increases water loss. Schematic, not to scale.Guard cells border the poreStomatal poreMore turgid → wider poreLess turgid → narrower poreCO₂ uptake trades off with water loss
A stoma is a pore between two guard cells. Changes in turgor alter the aperture; opening supports carbon-dioxide uptake but also increases water loss. Schematic, not to scale. Original teaching model.
Homeostasis model reference
FeatureMeaning
More turgidGuard cells bow apart; aperture widens
Less turgidPore narrows or closes
Trade-offCarbon-dioxide uptake and transpiration

Build the explanation

During water stress, abscisic acid (ABA) contributes to stomatal closure. ABA signalling in guard cells increases cytosolic calcium ions, which act as a second messenger. The signalling response promotes ion loss from guard cells; water follows by osmosis, turgor falls and the pore closes. This reduces transpiration and conserves water, although carbon-dioxide uptake may also decrease.

Apply the limits

ABA is a signal and calcium ions are a second messenger inside guard cells. The net response is reduced turgor and closure. Do not present ABA as a pore plug or claim closure eliminates all water loss.

Pause and explain

Explain one benefit and one possible cost of ABA-related stomatal closure.

Put the idea to work

Worked example

What is the role of calcium ions in ABA-related stomatal closure?

Show the worked solution
  1. ABA signalling changes calcium-ion levels in guard cells.
  2. Calcium acts as an intracellular second messenger that helps trigger the response.

Answer Calcium ions relay ABA signalling inside guard cells and contribute to ion loss and reduced turgor.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Trace the role of ABA and calcium ions in closure during water stress.

Give me a hint

Name the relevant structure or signal, then state its effect.

Compare my reasoning
  1. ABA signalling increases cytosolic calcium in guard cells.
  2. Ion and water loss reduces turgor, closing the pore.

During water stress, ABA signalling increases cytosolic calcium ions in guard cells. Calcium acts as a second messenger, promoting ion loss; water follows, turgor falls and the pore closes.

Look for these in your work

  • Correct mechanism
  • Direction of response explained
2 · Independent

A student calls calcium ions the hormone that travels from the root to close stomata. Correct the description.

Give me a hint

Separate the observed change from the body's response.

Compare my reasoning
  1. ABA is the signal associated with water stress.
  2. Calcium acts as a second messenger within guard cells.

Abscisic acid is the signalling molecule associated with water stress. Calcium ions act as a second messenger inside guard cells, helping relay the signal that leads to closure.

Look for these in your work

  • Evidence interpreted
  • Control step identified
3 · Transfer

Explain one benefit and one possible cost of ABA-related stomatal closure.

Give me a hint

Apply the mechanism to the new context without claiming more than the evidence shows.

Compare my reasoning
  1. Reduced aperture limits transpiration and conserves water.
  2. Less CO₂ may diffuse into the leaf, constraining photosynthesis.

Closure reduces water-vapour loss and helps conserve water during stress. It may also reduce carbon-dioxide uptake, which can constrain photosynthesis.

Look for these in your work

  • New context applied
  • Limits preserved

Common mistakes

  • Calling calcium the external hormone
  • Saying ABA closes the pore without guard-cell signalling
Recall without your notes

What can you explain now?

Which signal increases during water stress to promote stomatal closure?

Compare with the explanation

Abscisic acid

ABA signalling involves calcium ions as a second messenger.

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Make it stick

How do ABA and calcium signalling change stomatal aperture?

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Course scope and assessment

Two hundred and fifty-nine new lessons map every published learning outcome in Cambridge International Biology 9700 Topics 1–19 for exams 2028–2030. Each topic lesson includes guided, independent and transfer practice. Fifteen additional practical-assessment lessons provide online preparation for Paper 3 and Paper 5 practical assessment skills. Online Paper 3 lessons cannot provide supervised laboratory competence. Full timed cumulative exam practice and human subject-teacher review remain outstanding, so this is not exam-readiness evidence.

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