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IGCSE · Cambridge (CIE) · 0654

Absorb nitrate and build plant proteins

Plants absorb nitrate ions and use their nitrogen to make amino acids and proteins.

Carbon cycle · Cambridge IGCSE 0654 · B18.3

Mapped to the named 0654 section using teaching from the corresponding single-science course. A 0654-specific review of every Core and Supplement objective is still in progress. Written practice is self-checked; it does not demonstrate supervised laboratory competence or award an official Cambridge grade.

Before you begin

  • Use the food-web pathway and distinguish matter cycling from energy flow.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

What nitrogen-containing soil ion do plants absorb in this syllabus route?

What you will learn

  • Trace nitrate ions into plants
  • Connect nitrate uptake with amino-acid and protein production

Plants take up nitrate ions

Plant roots absorb nitrate ions from the soil solution. Nitrate supplies nitrogen that can be incorporated into amino acids; amino acids are then joined to make plant proteins. This transfers nitrogen from inorganic soil compounds into organic plant material.

Do not say plants absorb proteins from soil and turn them into plant protein. The route in the syllabus is uptake of nitrate ions, followed by production of amino acids and proteins.

Connect the plant step to feeding

When an animal eats a plant, nitrogen in plant proteins can transfer into the animal. Dead plant material and waste later provide protein for decomposition. Plants do not absorb nitrogen gas directly from the atmosphere in this route; fixation processes first make nitrogen compounds.

Keep the sequence: fixation and nitrification supply soil nitrate, plant uptake brings nitrate into the plant, and amino-acid/protein production incorporates its nitrogen.

Sources for this lesson

Pause and explain

What products are made from absorbed nitrate nitrogen?

Put the idea to work

Worked example

A plant absorbs nitrate from soil. Describe the next two nitrogen-containing products named in the syllabus.

Show the worked solution
  1. Nitrate is absorbed by the plant.
  2. Nitrogen is used to produce amino acids.
  3. Amino acids are joined into proteins.

Answer The plant uses absorbed nitrate nitrogen to produce amino acids and then proteins.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Complete the plant pathway: soil nitrate → plant → ____ → ____.

Give me a hint

Name the organic molecules in order.

Compare my reasoning
  1. Nitrate nitrogen is used to make amino acids.
  2. Amino acids are joined into proteins.

Soil nitrate ions → plant amino acids → plant proteins.

Look for these in your work

  • Amino acid intermediate
  • Protein product
2 · Independent

Correct the sentence “Plants get protein by absorbing it from nitrate in the soil.”

Give me a hint

Separate ion uptake from protein synthesis.

Compare my reasoning
  1. Plants absorb nitrate ions.
  2. They use nitrate nitrogen to make amino acids and proteins.

Plants absorb nitrate ions from the soil and use the nitrogen to produce amino acids and proteins; they do not absorb protein from nitrate.

Look for these in your work

  • Uptake corrected
  • Synthesis described
3 · Transfer

A plant has absorbed nitrate ions. How can that nitrogen later reach a herbivore?

Give me a hint

Follow the food link.

Compare my reasoning
  1. The plant incorporates nitrogen into proteins.
  2. The herbivore feeds on the plant.

The plant uses nitrate nitrogen to make amino acids and proteins; feeding transfers that protein nitrogen to the herbivore.

Look for these in your work

  • Plant incorporation included
  • Feeding transfer stated

Common mistakes

  • Saying plants absorb nitrate-containing proteins
  • Skipping amino acids
  • Saying plant roots absorb N₂ gas directly
Recall without your notes

What can you explain now?

What nitrogen-containing soil ion do plants absorb in this syllabus route?

Compare with the explanation

Nitrate ions

The plant uptake step is nitrate absorption.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Trace nitrogen from nitrate ions in soil into a plant protein.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Sciences — Co-ordinated (Double)

Course scope and assessment

This 2028–2029 pathway maps all 152 detailed subject-content sections in the published 0654 syllabus to original teaching and staged practice. It adapts teaching from the related single-science pathways and excludes clear 0610-only topics such as classification systems, human excretion, tropic responses, food supply, population dynamics, detailed pollution and biotechnology. Some explanations may include wider single-science context. A 0654-specific objective-by-objective review, Core/Supplement audit, human subject-teacher review, supervised laboratory experience and cumulative assessment remain in progress. Written practice is self-checked and does not predict an official grade. The 2025–2027 syllabus has not been reconciled here; choose the 2028–2029 syllabus only.

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