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IGCSE · Cambridge (CIE) · 0654

Plan a reproducible investigation of discontinuous pea phenotypes

Define categories, sample without selecting outcomes and report unclassified specimens.

Variation · Cambridge IGCSE 0654 · B17.1

Mapped to the named 0654 section using teaching from the corresponding single-science course. A 0654-specific review of every Core and Supplement objective is still in progress. Written practice is self-checked; it does not demonstrate supervised laboratory competence or award an official Cambridge grade.

Before you begin

  • Recall genes, alleles, genotype and phenotype from the inheritance pathway. Use the stated organism, units and sample; the records in these lessons are invented.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

What should happen to an unclassifiable damaged specimen?

What you will learn

  • Use a supplied phenotype key to tally specimens
  • Report exclusions and limits of categorical evidence

Original teaching model. Measurements, categories, DNA fragments and comparison records are invented; assumptions and sample boundaries are stated.

Plan a reproducible investigation of discontinuous pea phenotypesClassify under a stated key, retain exclusions and compare proportions using their own totals.Valid mature-seed recordsVertical scale: valid phenotypecount1515Round1010WrinkledSample total: 25Counts describe these inventedrecords, not an entire population
Classify under a stated key, retain exclusions and compare proportions using their own totals.
Variation measurement and evidence key
FeatureMeaning
CategoriesRound / wrinkled under the supplied key
Valid sample25: round 15 / wrinkled 10
Unclassified example3 damaged; 28 examined in total
Evidence limitCounts alone do not establish the parental cross

Use a supplied phenotype key to tally specimens

To investigate discontinuous variation, choose a same-species feature with a defined supplied key, such as mature pea seed shape. State the categories round and wrinkled, the specimen stage and the decision features from the key. Use clearly identified safe specimens or suitable supplied photographs. The classification is an observation under that key; it does not automatically establish a dominant allele, the parent genotypes or a universal rule for damaged seeds.

Choose specimens from a stated frame without deliberately selecting only round seeds or a desired ratio. Tally each valid specimen once and include a total check. If a seed is damaged, immature or not classifiable using the key, record that fact separately and explain how it affects the valid-record denominator. Removing unclear records silently makes the reader unable to evaluate the sampling and decision method.

Report exclusions and limits of categorical evidence

Suppose a sample has twenty-five clearly classifiable seeds: fifteen round and ten wrinkled. The valid phenotype percentages are sixty and forty per cent, and the observed count ratio is three to two. These numbers describe the sample. Without a supplied parental cross and inheritance rule, they do not establish the expected offspring probability or prove a deviation from a predicted Mendelian ratio.

Use labelled separated bars or a category table to describe the results. Compare more samples using explicit denominators rather than the larger raw count alone. A sample with more round records can still have a smaller round percentage if it contains many more seeds in total. Written classification planning and invented records practise scientific reasoning; supervision, actual observation accuracy and practical competence remain separate assessment work.

Sources for this lesson

Pause and explain

Do observed phenotype counts alone establish parental genotypes?

Put the idea to work

Worked example

Describe fifteen round and ten wrinkled valid seeds in a twenty-five-seed sample.

Show the worked solution
  1. Check the total 15 + 10 = 25.
  2. Calculate 15/25 × 100 = 60% and 10/25 × 100 = 40%; simplify 15:10 to 3:2.

Answer The valid sample is 60% round and 40% wrinkled, with observed ratio 3:2. A parental cross is not established.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Calculate round and wrinkled percentages from 15 and 10 valid records.

Give me a hint

Use the 25-record denominator.

Compare my reasoning
  1. 15/25 × 100 = 60%.
  2. 10/25 × 100 = 40%.

Round 60%, wrinkled 40% of the valid sample.

Look for these in your work

  • Total 25
  • Both percentages labelled
2 · Independent

Three damaged seeds accompany those 25 valid records. Report examined, valid and unclassified totals and the denominator for the stated phenotype percentages.

Give me a hint

Examined and classified totals can differ.

Compare my reasoning
  1. 25 + 3 = 28 examined.
  2. Only 25 have valid supplied-key phenotypes.

28 examined, 25 validly classified, 3 unclassified; the given 60%/40% phenotype percentages use 25.

Look for these in your work

  • All three totals
  • Explicit valid-record denominator
3 · Transfer

Sample X has 15 round out of 25, while sample Y has 24 round out of 50. Which has the larger round proportion, and can the two raw counts settle it?

Give me a hint

Calculate both percentages first.

Compare my reasoning
  1. X: 15/25 = 60%.
  2. Y: 24/50 = 48%.

X has the larger round proportion, 60% versus 48%, despite fewer round records. Raw counts alone cannot settle a comparison with different totals.

Look for these in your work

  • Both sample denominators
  • Proportion distinguished from count

Common mistakes

  • Choosing only specimens with a desired phenotype
  • Hiding unclassified records
  • Inferring parental genotypes from one observed count ratio
Recall without your notes

What can you explain now?

Do observed phenotype counts alone establish parental genotypes?

Compare with the explanation

No; a cross and inheritance evidence are missing

A descriptive sample is not a complete genetic investigation.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Without your notes, use a supplied phenotype key to tally specimens and report exclusions and limits of categorical evidence. Keep units, the stated sample and the limits of the evidence clear.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Sciences — Co-ordinated (Double)

Course scope and assessment

This 2028–2029 pathway maps all 152 detailed subject-content sections in the published 0654 syllabus to original teaching and staged practice. It adapts teaching from the related single-science pathways and excludes clear 0610-only topics such as classification systems, human excretion, tropic responses, food supply, population dynamics, detailed pollution and biotechnology. Some explanations may include wider single-science context. A 0654-specific objective-by-objective review, Core/Supplement audit, human subject-teacher review, supervised laboratory experience and cumulative assessment remain in progress. Written practice is self-checked and does not predict an official grade. The 2025–2027 syllabus has not been reconciled here; choose the 2028–2029 syllabus only.

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