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IGCSE · Cambridge (CIE) · 0654

Trace starch through maltose to glucose

Locate maltase at the small-intestine epithelial membrane.

Chemical digestion · Cambridge IGCSE 0654 · B7.4

Mapped to the named 0654 section using teaching from the corresponding single-science course. A 0654-specific review of every Core and Supplement objective is still in progress. Written practice is self-checked; it does not demonstrate supervised laboratory competence or award an official Cambridge grade.

Before you begin

  • Recall large and small nutrient molecules, cell membranes and enzyme specificity.
  • Use the stated diagram keys and evidence; models are not to scale.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

What is the intermediate named in this pathway?

What you will learn

  • Locate maltase at the small-intestine epithelial membrane.
  • Distinguish surface enzyme action from absorption of its product.
Amylase breaks starch to maltose; maltase on small-intestine epithelial membranes breaks maltose to glucose. Producing glucose is distinct from absorbing it across the lining.Two named starch-digestionstepsStarchAmylase → maltoseMaltose at epithelial surfaceMembrane-associated maltaseGlucoseAvailable for absorption
Amylase breaks starch to maltose; maltase on small-intestine epithelial membranes breaks maltose to glucose. Producing glucose is distinct from absorbing it across the lining.

Use two enzyme steps

In the required Extended sequence, amylase breaks starch down to maltose. Maltase then breaks maltose down to glucose. A two-stage pathway needs the correct enzyme for each substrate; amylase and maltase are not synonyms.

The first step can occur in the mouth and small intestine through the amylase routes. The second step is associated with maltase on the membranes of the epithelium lining the small intestine.

Locate the membrane-associated action

The epithelial lining separates the gut contents from underlying tissues. Membrane-associated maltase acts at this surface; it is not simply a pancreas-secreted enzyme acting inside the stomach.

Glucose produced becomes available for absorption. Making glucose and transporting it across the lining are distinct events. The diagram shows the required pathway, not every other starch product or transport protein.

Sources for this lesson

Pause and explain

Which location fits maltase?

Put the idea to work

Worked example

A pathway shows starch —amylase→ maltose —?→ glucose beside the epithelial membrane. Complete and locate the enzyme.

Show the worked solution
  1. The second substrate is maltose.
  2. Choose maltase.
  3. Place it at small-intestine epithelial membranes.

Answer Maltase on membranes of the small-intestine epithelium catalyses maltose breakdown to glucose.

Try a topic test

Choose Easy, Normal or Difficult. Save your answers and open worked review after finishing.

Test this topic →

From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Write the starch → maltose → glucose pathway with both enzymes.

Give me a hint

Use one enzyme above each step.

Compare my reasoning
  1. Amylase: starch to maltose.
  2. Maltase: maltose to glucose.

Starch —amylase→ maltose —maltase→ glucose.

Look for these in your work

  • Correct intermediate.
  • Both enzymes correctly placed.
2 · Independent

Correct a label that places maltase secretion by the pancreas as the required site.

Give me a hint

The syllabus specifies membrane-associated action.

Compare my reasoning
  1. Maltase is on small-intestine epithelial membranes.
  2. Pancreatic amylase has a separate route.

Place maltase at the epithelial membranes lining the small intestine.

Look for these in your work

  • Correct location.
  • No amylase/maltase swap.
3 · Transfer

A mixture forms maltose but no glucose is detected under the stated test conditions. Which named step needs investigation next?

Give me a hint

The second product is missing.

Compare my reasoning
  1. First step produced maltose.
  2. Investigate maltase and suitable conditions.
  3. Check the glucose measurement before inferring failure.

Investigate the maltase step and the glucose test, rather than assuming amylase must perform both stages.

Look for these in your work

  • Second-step logic.
  • Measurement control.

Common mistakes

  • Amylase does not perform the named maltase step.
  • Membrane enzyme action is not the same as glucose transport.
Recall without your notes

What can you explain now?

Which location fits maltase?

Compare with the explanation

Membranes of the small-intestine epithelium.

It is associated with the lining surface.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Draw both reactions and the epithelial-membrane location of the second.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Sciences — Co-ordinated (Double)

Course scope and assessment

This 2028–2029 pathway maps all 152 detailed subject-content sections in the published 0654 syllabus to original teaching and staged practice. It adapts teaching from the related single-science pathways and excludes clear 0610-only topics such as classification systems, human excretion, tropic responses, food supply, population dynamics, detailed pollution and biotechnology. Some explanations may include wider single-science context. A 0654-specific objective-by-objective review, Core/Supplement audit, human subject-teacher review, supervised laboratory experience and cumulative assessment remain in progress. Written practice is self-checked and does not predict an official grade. The 2025–2027 syllabus has not been reconciled here; choose the 2028–2029 syllabus only.

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