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IGCSE · Cambridge (CIE) · 0970

Separate predicted monohybrid ratios from observed offspring counts

Predictions, observations and numerical departures answer different questions.

Monohybrid inheritance pathway · 0970 (9–1) · 17.4
Exam scope and assessment notes

Core and Extended · Biology 0970, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked; human subject-teacher review, supervised practical competence and cumulative assessment remain pending. Cambridge states that IGCSE 0970 has the same subject content as IGCSE 0610, with 9–1 grading. This StudyForA pathway maps the 2026–2028 syllabus only. This 9–1 graded qualification is available only in a limited number of administrative zones. Confirm that your centre can enter you for this code and exam series with Cambridge International. Cambridge has published a separate syllabus for 2029. StudyForA has not reconciled that version yet; this page supports exams in 2026, 2027 and 2028 only.

Before you begin

  • Recall chromosomes, genes and alleles, meiosis, haploid gametes and fertilisation restoring two chromosome sets.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

What denominator is used for 62 pigment-present records among 80 offspring?

What you will learn

  • Calculate model expectations and observed percentages
  • Use observations without treating a finite sample as proof of one cross

Original teaching model. Pedigrees and records are fictional; allele and probability assumptions are stated.

Separate predicted monohybrid ratios from observed offspring countsPredictions, observations and numerical departures answer different questions.Invented sample compared with expectationTotal: 80 offspringPigment presentExpected60Observed62Observed: 77.5% of totalPigment absentExpected20Observed18Observed: 22.5% of totalPigment-present departure: 2.5percentage pointsInvented sample; a departure alonedoes not establish its cause
Predictions, observations and numerical departures answer different questions.
Allele, phenotype and evidence key
FeatureMeaning
3:1 expected total 8060 present, 20 absent
Invented observed total 8062 present, 18 absent
Observed percentages77.5% present; 22.5% absent
Departure2.5 percentage points; not proof of a causal mechanism

Calculate model expectations and observed percentages

Predicted ratios come from the parental genotypes, gamete possibilities and expression rule. Under complete dominance, Tt × Tt predicts a 3:1 pigment phenotype ratio and Tt × tt predicts 1:1. These are probabilities under the stipulated normal model. An observed ratio comes from counting the actual supplied offspring records; it describes that sample rather than automatically establishing its parents.

For a predicted total of eighty from Tt × Tt, three quarters gives sixty pigment-present and one quarter gives twenty absent. An invented record of sixty-two present and eighteen absent still totals eighty. Its observed present percentage is sixty-two divided by eighty times one hundred, seventy-seven point five per cent; its absent percentage is twenty-two point five per cent.

Use observations without treating a finite sample as proof of one cross

The observed present percentage is two point five percentage points above the seventy-five per cent model expectation. This numerical departure is not by itself proof that the model is false, that a mutation occurred or that a particular parent has a different genotype. Chance sampling and the biological assumptions need to be considered; formal statistical testing is outside this lesson’s requirement.

Keep the denominator and wording clear. Expected sixty and twenty are not the recorded sixty-two and eighteen. The next offspring’s probability in the stipulated independent model does not change merely to force the final sample into a perfect ratio. If a task supplies uncertain parent genotypes, state the compatible possibilities and evidence limits rather than declaring certainty from a small observed ratio.

Pause and explain

Does a predicted 3:1 ratio guarantee exact matching counts in every finite sample?

Put the idea to work

Worked example

Compare an 80-offspring 3:1 prediction with invented observed counts of 62 pigment-present and 18 absent.

Show the worked solution
  1. Multiply 80 by 3/4 and 1/4 to obtain expected counts 60 and 20.
  2. Divide the observations by 80 and multiply by 100: 77.5% and 22.5%. Pigment presence is 77.5 − 75 = 2.5 percentage points above the predicted fraction.

Answer Expected for 80 at 3:1: 60 present, 20 absent. Invented observations 62/18 give 77.5%/22.5%, a 2.5 percentage-point departure for pigment presence.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Calculate expected present and absent counts for eighty offspring under a 3:1 phenotype prediction.

Give me a hint

Use three quarters and one quarter of the total.

Compare my reasoning
  1. Calculate 80 × 3/4.
  2. Calculate 80 × 1/4.

Expected present 60 and absent 20 under the model.

Look for these in your work

  • Correct sixty and twenty
  • Expected rather than observed wording
2 · Independent

An invented sample has 62 present and 18 absent among 80. Calculate both percentages and the present percentage-point departure from 75%.

Give me a hint

Use eighty for both percentages.

Compare my reasoning
  1. Calculate 62/80 × 100.
  2. Calculate 18/80 × 100.
  3. Subtract 75 from the observed present percentage.

Present 77.5%, absent 22.5%; present is 2.5 percentage points above the 75% model expectation.

Look for these in your work

  • Correct percentages
  • Correct 2.5 percentage-point departure
3 · Transfer

A small sample from a known Tt × tt cross happens to show more pigment-present offspring. A student changes the next-offspring absence probability to compensate. Explain the error under the stated independent model.

Give me a hint

Previous observations do not impose a balancing rule.

Compare my reasoning
  1. Retain the known parental genotypes.
  2. Use one-half for each phenotype in the model.
  3. Distinguish finite observations from forced compensation.

The model still gives absence probability 1/2 for the next offspring. Earlier sample imbalance does not require compensation when the events are stipulated independent; a small observed departure does not change the known cross.

Look for these in your work

  • Keeps one-half probability
  • Rejects forced balancing from previous outcomes

Common mistakes

  • Calling recorded counts the exact model prediction
  • Using a departure alone to diagnose an inheritance mechanism
  • Changing the next-event probability to force a perfect ratio
Recall without your notes

What can you explain now?

Does a predicted 3:1 ratio guarantee exact matching counts in every finite sample?

Compare with the explanation

No

It supplies probabilities under assumptions, while observed samples can differ.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Without your notes, calculate model expectations and observed percentages and use observations without treating a finite sample as proof of one cross. Use the supplied allele key and state what the evidence can establish.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology (9-1)

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked. This is the separate 9–1 qualification 0970; Cambridge states that its 2026–2028 subject content is otherwise the same as 0610. This 9–1 graded qualification is available only in a limited number of administrative zones. Confirm that your centre can enter you for this code and exam series with Cambridge International. The 2029 syllabus is separately published at https://www.cambridgeinternational.org/Images/764352-2029-syllabus.pdf and is not yet mapped here. This course supports exams in 2026, 2027 and 2028 only.

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