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IGCSE · Cambridge (CIE) · 0610

Choose between diffusion osmosis and active transport from evidence

Use substance, boundary, gradient and energy to justify a mechanism.

Active transport pathway · 3.3

Extended only (Supplement); Biology 0610, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked

Before you begin

  • Recall Core definitions plus Extended water potential and carriers.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

Only an inward arrow is given. What is the best response?

What you will learn

  • Use substance, boundary, gradient and energy to justify a mechanism.
  • Apply the idea to a supplied biological example or investigation.
Diffusion follows the particle concentration gradient; osmosis concerns water and water potential across a partially permeable membrane; active transport uses respiratory energy and carriers for the stated against-gradient transfer. Inward arrows alone are insufficient.Choose the mechanism fromevidenceDiffusionParticles: higher → lowerRandom kinetic motionOsmosisWater: higher → lowerpotentialPartially permeable membraneActive transportParticles: lower → higherRespiration + protein carriers
Diffusion follows the particle concentration gradient; osmosis concerns water and water potential across a partially permeable membrane; active transport uses respiratory energy and carriers for the stated against-gradient transfer. Inward arrows alone are insufficient.

Ask four questions

First identify the moving substance: water, a gas or a solute. Then identify the boundary and compare the relevant concentrations or water potentials. Finally ask whether respiratory energy is supplied for against-gradient transfer. A mechanism name should follow evidence rather than a memorised arrow direction.

Diffusion gives net movement down a concentration gradient from random kinetic motion. Osmosis concerns water through a partially permeable membrane, from higher to lower water potential. Active transport uses respiratory energy and membrane protein carriers for the stated against-gradient particle transfer.

Use only the evidence provided

Inward arrows can represent all three processes in different conditions. A question that gives only an inward arrow lacks enough information to distinguish them. State what comparison or energy evidence is needed instead of guessing.

The processes can coexist across one membrane. Oxygen can diffuse while ions are actively accumulated and water crosses by osmosis. They do not have to share the same gradient, direction or energy requirement. This is an Extended integration lesson, building on the Core diffusion and active-transport definitions.

Pause and explain

Which pairing is correct?

Put the idea to work

Worked example

A cell takes in oxygen from higher external oxygen concentration, water from higher external water potential, and an ion against its gradient using a carrier and respiratory energy. Name the three processes.

Show the worked solution
  1. Oxygen follows its concentration gradient: diffusion.
  2. Water follows water potential across the membrane: osmosis.
  3. The carrier transfers the ion against its gradient using energy: active transport.

Answer Oxygen: diffusion; water: osmosis; ion: active transport.

Try a topic test

Choose Easy, Normal or Difficult. Save your answers and open worked review after finishing.

Test this topic →

From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

An ion enters from lower external to higher internal concentration using a carrier and respiration. Justify the mechanism.

Give me a hint

Apply all active criteria.

Compare my reasoning
  1. Against-gradient ion movement.
  2. Carrier and respiratory energy supplied.

Active transport.

Look for these in your work

  • Use direction, carrier and energy.
2 · Independent

Water enters through a partially permeable membrane from higher outside water potential. Justify the mechanism.

Give me a hint

The moving substance is water.

Compare my reasoning
  1. Water crosses the required membrane.
  2. Net direction is higher to lower water potential.

Osmosis.

Look for these in your work

  • Give substance, membrane and comparison.
3 · Transfer

A diagram shows only a dissolved substance moving out. Give two pieces of missing evidence needed to separate diffusion from active transport.

Give me a hint

Compare concentrations and energy.

Compare my reasoning
  1. Ask the substance concentrations on each side.
  2. Ask whether respiratory energy is supplied for transfer.

Its gradient direction and energy requirement; membrane/carrier information can also help.

Look for these in your work

  • Do not infer mechanism from outward direction alone.

Common mistakes

  • An arrow alone cannot name the mechanism.
  • Different substances can cross the same membrane differently.
Recall without your notes

What can you explain now?

Which pairing is correct?

Compare with the explanation

Osmosis: water potential; active ion uptake: ion concentration and respiratory energy.

Use the appropriate driving comparison.

After trying it yourself, choose your next review. This is your self-assessment.

Your review choice appears on Today. Sign in to sync it across devices.

Make it stick

Classify three new examples using evidence rather than arrow orientation.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked.

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