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IGCSE · Cambridge (CIE) · 0610

Use base-pair totals to check a DNA model

Calculate paired-molecule counts without assuming single-strand equality.

Biological molecules pathway · 4.1

Extended only (Supplement); Biology 0610, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked

Before you begin

  • Recall the distinction between an element, a molecule and a cell structure.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

How many bases occur in 15 base pairs?

What you will learn

  • Calculate paired-molecule counts without assuming single-strand equality.
  • Explain the conclusion from stated evidence and recognise its limits.
A paired DNA segment with 40 bases including 12 A has 12 T, eight C and eight G. The sums count both strands, not one isolated strand. Equal totals do not establish identical base order or ancestry.Count the whole paired molecule40 bases across both strandsA = 12, so T = 12Remaining: 40 − 24 = 16C = 8, so G = 8Check the whole total12 + 12 + 8 + 8 = 4020 pairs contain 40 basesCounts do not reveal base order
A paired DNA segment with 40 bases including 12 A has 12 T, eight C and eight G. The sums count both strands, not one isolated strand. Equal totals do not establish identical base order or ancestry.

Count both strands

Each base pair contains two bases. A double-stranded segment with 24 base pairs therefore contains 48 bases in total. The total A count equals total T, and total C equals total G in the required paired model.

Use the stated whole-molecule scope. If total A is supplied, total T matches it; the remaining bases are divided equally between C and G. These calculations are supporting practice in pairing, not a separate syllabus demand for DNA percentage formulae.

Recognise unsupported claims

A and T do not each have to be 25% of every DNA molecule; different paired molecules can have different overall compositions. Equality within each complementary pair leaves many possible compositions.

Knowing total composition does not reveal the exact base sequence or prove a relationship between organisms. Counts ignore order. Classification comparisons require aligned sequence evidence, not just matching totals.

Pause and explain

Do matching overall base counts prove two sequences are identical?

Put the idea to work

Worked example

A paired DNA segment contains 40 bases total, with 12 A. Find T, C and G totals.

Show the worked solution
  1. T = 12 because A pairs with T.
  2. Remaining bases = 40 − 24 = 16.
  3. C and G share those equally.

Answer T = 12, C = 8 and G = 8.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

How many bases are in 18 base pairs?

Give me a hint

Two per pair.

Compare my reasoning
  1. 18 × 2 = 36.

36 bases.

Look for these in your work

  • Include the named molecules or components in your answer.
  • Explain the relationship using the stated building blocks or paired-base evidence.
2 · Independent

A paired segment has 60 bases including 18 C. Find G, A and T.

Give me a hint

Match C and G then divide the remainder.

Compare my reasoning
  1. G = 18.
  2. 60 − 36 = 24 remain.
  3. A = T = 12.

G = 18, A = 12 and T = 12.

Look for these in your work

  • Include the named molecules or components in your answer.
  • Explain the relationship using the stated building blocks or paired-base evidence.
3 · Transfer

Two segments have identical base totals. What extra evidence is needed to establish identical sequence?

Give me a hint

Restore the missing ordering information.

Compare my reasoning
  1. Compare aligned base order at each position.
  2. Equal counts alone are insufficient.

The aligned sequence of bases, not just totals.

Look for these in your work

  • Include the named molecules or components in your answer.
  • Explain the relationship using the stated building blocks or paired-base evidence.

Common mistakes

  • Different arrangements can have matching counts.
  • Complementary equality does not fix those percentages.
Recall without your notes

What can you explain now?

Do matching overall base counts prove two sequences are identical?

Compare with the explanation

No, base order can differ.

Counts do not preserve sequence order.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Check a new paired-molecule count using equality and the total sum.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked.

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