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IGCSE · Cambridge (CIE) · 0610

Investigate biological washing powders

Biological washing powders contain enzymes that break down biological stains; the investigation should compare cleaning under controlled conditions.

Biotechnology applications pathway · 21.2

Core and Extended; Biology 0610, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked

Before you begin

  • Know that genes are sections of DNA and that organisms use enzymes in cellular processes.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

Which enzyme can break down a protein stain?

What you will learn

  • Describe the use of enzyme-containing biological washing powders
  • Plan a fair test that investigates cleaning effectiveness

Let enzymes break down stains

Biological washing powders contain enzymes that act on biological stains. Proteases break down proteins, lipases break down fats and amylases break down starch. Smaller soluble products can then be removed from fabric during washing.

Different stains contain different materials, so the relevant enzyme matters. Enzymes work best within suitable temperature and pH ranges; an excessively high temperature can denature them and reduce their activity.

Design a controlled comparison

To compare a biological powder with a non-biological powder, use equal fabric pieces with the same stain, stain amount, drying time, detergent concentration, water volume, temperature and wash duration. A no-detergent control can show how much cleaning happens without detergent.

Measure stain removal consistently, for example by image brightness under fixed lighting or a defined colour scale. Repeat trials to identify variation. Keep the conclusion within the tested stain and wash conditions.

Pause and explain

Why compare detergent powders using the same wash temperature?

Put the idea to work

Worked example

A protein stain is washed with biological powder at a suitable temperature. Which enzyme acts on the stain, and what is its role?

Show the worked solution
  1. Identify the stain material.
  2. Match the enzyme to its substrate.

Answer Protease acts on the protein stain and breaks proteins into smaller soluble products that can be washed away.

Try a topic test

Choose Easy, Normal or Difficult. Save your answers and open worked review after finishing.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Match each stain with a suitable enzyme: protein, fat and starch.

Give me a hint

Recall enzyme substrate specificity.

Compare my reasoning
  1. Protease acts on protein.
  2. Lipase acts on fat; amylase acts on starch.

Protease acts on protein, lipase on fat and amylase on starch.

Look for these in your work

  • All three matches correct
  • Substrates distinguished
2 · Independent

Describe a fair test of whether a biological powder removes a protein stain more effectively than a non-biological powder.

Give me a hint

Change powder type while matching the stain and wash conditions.

Compare my reasoning
  1. Use identical fabric pieces with the same protein stain and stain amount.
  2. Keep powder concentration, volume, temperature, time and measurement method the same; repeat.

Use identical stained fabric pieces and wash each under the same conditions, changing only the powder type. Measure stain removal consistently and repeat the test to assess variation.

Look for these in your work

  • One variable changed
  • Controls and measurement included
3 · Transfer

An enzyme powder cleans a protein stain poorly in very hot water. Explain a possible reason.

Give me a hint

Consider how temperature affects enzyme structure.

Compare my reasoning
  1. High temperature can alter enzyme shape.
  2. The substrate may no longer fit the active site effectively.

Very high temperature can denature the protease, changing its active site so it breaks down the protein stain less effectively.

Look for these in your work

  • Temperature effect stated
  • Enzyme activity linked

Common mistakes

  • Saying enzymes are used up as the stain
  • Using a temperature that denatures the enzyme
  • Comparing fabrics with different stain amounts or wash times
Recall without your notes

What can you explain now?

Which enzyme can break down a protein stain?

Compare with the explanation

Protease

Proteases act on proteins.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Which enzyme acts on each common biological stain, and what must remain controlled in a washing-powder test?

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked.

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