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IGCSE · Cambridge (CIE) · 0610

Nitrify ammonium into nitrate

Nitrifying microorganisms convert ammonium compounds into nitrate ions, making a form of nitrogen available for plant uptake.

Nutrient cycles pathway · 19.3

Extended only (Supplement); Biology 0610, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked

Before you begin

  • Use the food-web pathway and distinguish matter cycling from energy flow.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

What is the main nitrogen-cycle change in nitrification?

What you will learn

  • Describe nitrification in the nitrogen cycle
  • Distinguish nitrification from fixation and denitrification

Nitrification changes soil nitrogen compounds

Nitrifying microorganisms convert ammonium compounds into nitrate ions through nitrification. In a more detailed account, intermediate nitrogen compounds may be present, but the key syllabus route is ammonium to nitrate.

Nitrification does not take atmospheric N₂ and does not return nitrogen to the air. The process changes nitrogen already in soil compounds. Individual microorganisms do not need to be named for this objective.

Use the correct direction

The cycle route is ammonium compounds → nitrate ions by nitrification. Plants can absorb nitrate ions; other microorganisms can denitrify nitrate back to nitrogen gas. Trace each arrow by checking its starting and ending compounds.

Do not confuse nitrification with nitrogen fixation. Fixation introduces atmospheric nitrogen into compounds; nitrification transforms soil ammonium compounds into nitrates.

Pause and explain

Which role do nitrifying microorganisms perform?

Put the idea to work

Worked example

Soil contains ammonium ions. A microorganism process produces nitrate ions. Name the process.

Show the worked solution
  1. The starting form is ammonium.
  2. The product is nitrate.

Answer The process is nitrification.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

Complete the route: ammonium compounds → ____ ions by _____.

Give me a hint

Name the product and process.

Compare my reasoning
  1. The product taken up by plants is nitrate.
  2. Ammonium to nitrate is nitrification.

Ammonium compounds → nitrate ions by nitrification.

Look for these in your work

  • Product correct
  • Process correct
2 · Independent

A learner says nitrifying microorganisms return nitrogen to the atmosphere. Correct the statement.

Give me a hint

Identify the product of nitrification.

Compare my reasoning
  1. Nitrification produces nitrate ions from ammonium compounds.
  2. Denitrification returns nitrate nitrogen to N₂ gas.

Nitrifying microorganisms convert ammonium compounds into nitrate ions. Denitrification, not nitrification, returns nitrate nitrogen to atmospheric gas.

Look for these in your work

  • Nitrification role correct
  • Denitrification distinguished
3 · Transfer

A sample has nitrate ions after soil microorganisms act on ammonium. What process is supported, and what organism-specific claim is unnecessary?

Give me a hint

Use the chemical change and the syllabus wording.

Compare my reasoning
  1. Ammonium to nitrate supports nitrification.
  2. The syllabus uses generic microorganism names.

The change supports nitrification. It is unnecessary to name a particular bacterial genus; the syllabus requires the generic microorganism role.

Look for these in your work

  • Process inferred from compounds
  • Unrequired naming avoided

Common mistakes

  • Reversing ammonium and nitrate
  • Calling nitrification fixation
  • Claiming nitrification directly produces amino acids
Recall without your notes

What can you explain now?

What is the main nitrogen-cycle change in nitrification?

Compare with the explanation

Ammonium compounds are converted to nitrate ions

This names the direction required.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

State the direction of nitrification and distinguish it from nitrogen fixation and denitrification.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked.

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