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IGCSE · Cambridge (CIE) · 0610

Investigate plant tissue in different solution concentrations

Plan a fair concentration comparison and describe changes in mass and firmness.

Osmosis pathway · 3.2

Core and Extended; Biology 0610, 2026–2028. Use the stated specimen features and evidence. Diagrams are schematic. Written practice is self-checked

Before you begin

  • Recall cell membranes and osmosis.

Work on paper. Try each question before revealing help, and explain why your method works.

Quick readiness check

In a solution-concentration investigation, what should be varied?

What you will learn

  • Plan a fair concentration comparison and describe changes in mass and firmness.
  • Apply the idea to a supplied biological example or investigation.
Comparable plant-tissue samples are exposed to different solution concentrations. Time, temperature, tissue source, dimensions and blotting are controlled. Written plans and supplied data do not certify supervised practical competence.Matched tissue, variedconcentrationLowMediumHighVary solution concentrationControl the comparisonSame time, temperature andtissueConsistent blotting · repeat
Comparable plant-tissue samples are exposed to different solution concentrations. Time, temperature, tissue source, dimensions and blotting are controlled. Written plans and supplied data do not certify supervised practical competence.

Set up a matched comparison

Comparable pieces of plant tissue, such as potato cylinders, can be immersed in a range of known solution concentrations. Concentration is the independent variable; mass change over a fixed interval is a possible dependent variable. Use tissue from a comparable source with matched starting dimensions, temperature, solution volume and immersion time.

Measure initial and final mass using the same balance and consistent gentle blotting. Repeat each concentration. Percentage change makes comparisons between slightly different initial masses more useful, but it does not remove every difference in tissue condition or surface exposure.

Describe before explaining

In a simple model with retained solute, more dilute surroundings may produce water entry, mass gain and greater firmness. More concentrated surroundings may cause water loss, mass loss and reduced firmness. Describe the supplied observations first, then explain them using osmosis.

Plant tissue is made of many cells, and a tissue-level measurement does not directly show every membrane or the condition of every cell. Extended learners use water potential and cell-state terminology in later lessons. These plans and sample results remain preparation for supervised practical work.

Pause and explain

What does a tissue mass loss show directly?

Put the idea to work

Worked example

A plant cylinder is 4.0 g initially and 4.4 g after immersion. Describe the mass change.

Show the worked solution
  1. Subtract initial from final.
  2. 4.4 − 4.0 = 0.4 g.
  3. The positive change is a gain; water entry is a possible explanation under the model.

Answer A 0.4 g mass gain.

Try a topic test

Choose Easy, Normal or Difficult. Save your answers and open worked review after finishing.

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From guided practice to a new situation

Write your answer and reasoning first. Use a hint only if you are stuck. The model solution is for self-checking; your written work is not automatically marked.

1 · Guided

A cylinder changes from 3.5 g to 3.15 g. Describe its mass change.

Give me a hint

Final minus initial.

Compare my reasoning
  1. 3.15 − 3.5 = −0.35 g.
  2. It lost mass.

A 0.35 g loss.

Look for these in your work

  • Give magnitude and sign.
2 · Independent

Name three controls when comparing concentrations.

Give me a hint

Keep tissue and exposure conditions comparable.

Compare my reasoning
  1. Match tissue source and dimensions.
  2. Use the same immersion time and temperature.

Any three of tissue source/dimensions, time, temperature, solution volume and blotting method.

Look for these in your work

  • Choose controlled variables, not concentration.
3 · Transfer

Why is using one damaged cylinder only in the strongest solution a problem?

Give me a hint

Consider another cause of water or mass loss.

Compare my reasoning
  1. Damage may alter membranes or cause leakage.
  2. Use comparable undamaged tissue and repeats.

Tissue damage confounds concentration; use matched tissue and repeats.

Look for these in your work

  • Identify the confound.

Common mistakes

  • Describe observations separately from inferred mechanism.
  • Percentage adjustment does not replace experimental controls.
Recall without your notes

What can you explain now?

What does a tissue mass loss show directly?

Compare with the explanation

The measured tissue became lighter.

Net water loss is an interpretation using the stated conditions.

After trying it yourself, choose your next review. This is your self-assessment.

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Make it stick

Write a fair plant-tissue concentration comparison.

If you needed the solution, close it and solve the problem again from a blank page. A correct answer is stronger when you can explain the reason for each step.

Keep learning Biology

Course scope and assessment

780 sequenced lessons provide 2340 guided, independent and application problems in characteristics, classification, cells, organisation, specimen size, membrane transport, biological molecules, enzymes, plant and human nutrition, plant transport, circulatory systems, the heart, blood vessels, blood, disease transmission, body defences, immunity, gas exchange in humans, respiration energy uses and investigations, aerobic respiration, anaerobic respiration and recovery, excretion in humans, coordination and response, sense organs, hormones, homeostasis, tropic responses, drugs, asexual reproduction, sexual reproduction, sexual reproduction in plants, sexual reproduction in humans, sex hormones in humans, sexually transmitted infections, chromosomes, genes and proteins, mitosis, meiosis, monohybrid inheritance, variation, adaptive features, selection, energy flow, food chains and webs, nutrient cycles, population, community, ecosystem, population-growth phases, food supply, habitat destruction, pollution, conservation, biotechnology and genetic modification. 61 of 61 syllabus sections have linked teaching and staged practice. The earlier inheritance overview remains introductory, and the monohybrid pathway develops the relevant Core and Supplement requirements. This is not full programme completion or guaranteed exam readiness. Written practice is self-checked.

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